Determination Of Eight Bound Nitrofuran Residues, Using A Rapid Microwave-Assisted Sample Preparation Approach With UHPLC-MS/MS Detection – Webinar Video

Speaker : Dr. Martin Danaher

Nitrofurans are a class of antibacterial agents that are banned from use in food producing animals on several continents, due to their undesirable toxicological properties. Methodology for analysing these banned compounds is standard in most countries, with analysis primarily focusing on four main compounds, detected as their marker residues, AOZ, AMOZ, AHD and SEM. Analysis of nitrofurans using the bound residue approach provides the most sensitive and selective detection, but it is time consuming and leads to longer sample turnaround times. The aim of this work was to extend the scope of analysis and develop a high throughput method to include four additional nitrofuran compounds, detected as their markers dinitro-salicylic acid hydrazine (DNSAH), hydroxybenzhydrazide (HBH), oxamic acid hydrazide (OAH) and aminoguanidine (AG). The analysis time was shortened from 4 days to 1.5 days by developing a rapid sample preparation approach, using a microwave-assisted derivatisation step and a modified QuEChERS-based extraction. The limits of quantification (LOQ) for all analytes ranged from ranged from 0.02 to 0.04 µg kg-1, with the exception of semicarbazide and aminoguanidine (LOQ = 0.2 µg kg-1).

 About the Speaker

Dr. Martin Danaher is a Principal Research Officer based at the Teagasc Food Research Centre in Dublin, Ireland. He has over 20 years’ experience in the field of analytical chemistry and specialises in the application of separation science in the area of food analysis. He is head of the national reference laboratory for certain veterinary drug and pesticide residues in foods of animal origin. He is also lead research scientist in the area chemical analysis at the Teagasc Dublin food research centre. His
research work extends to other areas of food analysis including mycotoxins, plant toxins, biocides, carbohydrates, vitamins and minerals. He leads a team of approximately 20 scientists made up of PhD students, research scientists and technologists. Dr. Danaher has coordinated or collaborated on over 30 research projects and published >100 peer reviewed papers and book chapters